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KMID : 1177720080020020021
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Journal of Alternatives to Animal Experiments
2008 Volume.2 No. 2 p.21 ~ p.33
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Internal Establishment and Validation of OECD Guidelines of Acute Oral Toxicity
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Cho Young-Rae
Yum Young-Na
Han Eui-Sik
Kwack Seung-Jun
Kim Hyung-Sub
Kang Mi-Sun
Lee Jin-Young
Oh Jae-Ho
Lim Chae-Hyung
Kim Dae-Seong
Kim Seung-Hee
Kang Tae-Seok
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Abstract
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As the study about the non-animal tests came to be internationally active and the interest in the animal welfare gradually became increased, OECD TG (Test Guideline) 401 that has been used since 1987 was abolished in 2002. Because TG401 is acute oral toxicity method depending on survival and death of many animals, it was heavily criticized. Therefore, the three alternative methods were developed. OECD TG420 and TG423 determine the class of chemicals according to GHS (Globally Harmonized Classification System for Chemical Substances and Mixtures) classification and OECD TG425 suggests the predicted LD50 of chemicals using AOT425 program. In this study, 10 chemicals were selected. The internationally admitted TG420, TG423 and TG425 were introduced and established through the method that these chemicals were orally administrated to SD female rats and then, the results were observed. Each chemical belonged to already known GHS class in the study using TG420 and TG423 and predicted LD50 was same or higher in the study using TG425 compared to already known LD50 value. In conclusion, the result of our study confirmed the decrease in the animal number and validated. The international harmonization of the non-animal tests will be pursued through this validation study.
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KEYWORD
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Acute oral toxicity test, test guideline, GHS classification, LD50
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